Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antiserum

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Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antiserum.

Detection of antibody to Coccidioides immitis F antigen is of proved value in the diagnosis of coccidioidomycosis. This antibody is demonstrable by use of an immunodiffusion assay with reference coccidioidin antigen and antiserum to C. immitis. Using a combination of lectin affinity and immunoaffinity chromatography, we isolated the F antigen from coccidioidin and prepared monospecific antibody...

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Immunoaffinity isolation and partial characterization of the Coccidioides immitis antigen detected by the tube precipitin and immunodiffusion-tube precipitin tests.

The antigen participating in the tube precipitin (TP) serologic test for coccidioidomycosis was isolated from mycelial-phase antigen (coccidioidin) by immunoaffinity and characterized by various analytical procedures. This was accomplished by first preparing the antigen-antibody precipitate by using antigen and human serum positive for TP (immunoglobulin M) antibody and then liberating the anti...

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Isolation of antigens with proteolytic activity from Coccidioides immitis.

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isolation and purification of hla-dr antigen from daudi cell line by immunoaffinity chromatography

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1987

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.55.1.227-232.1987